Product datasheet
Anti-PLGF antibody ab83906
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Overview
Product name
Anti-PLGF antibody
Description
Rabbit polyclonal to PLGF
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PLGF.Read
Abcam's proprietary immunogen policy(Peptide available as ab95428.)
Positive control
This antibody gave a positive signal in Human Placenta Tissue Lysate, and JEG-3, SW480 and
RAW 264.7 whole cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab83906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa
(predicted molecular weight: 26 kDa).Can be blocked with Human PLGF peptide
(ab95428).
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Target
Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation
and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1
and NRP2/neuropilin-2 in a heparin-dependent manner.
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8
week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Post-translational
modifications
N-glycosylated.
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless
released by heparin.
Anti-PLGF antibody images
All lanes : Anti-PLGF antibody (ab83906) at
1 µg/ml
Lane 1 : Placenta (Human) Tissue Lysate adult normal tissue (ab29745)
Lane 2 : JEG-3 (Human placental
choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon
adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic
monocyte macrophage cell line) Whole Cell
Western blot - PLGF antibody (ab83906)
Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 55 kDa
Exposure time : 4 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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