Product datasheet
Anti-Glutathione Peroxidase 1 antibody ab21966
1 Abreviews 4 References 2 Images
Overview
Product name
Anti-Glutathione Peroxidase 1 antibody
Description
Sheep polyclonal to Glutathione Peroxidase 1
Tested applications
Dot Blot, IHC-P, IHC-Fr, WB, ELISA
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
Immunogen
Synthetic peptide:
RRYSRRFQTIDIEPDIEALL
, corresponding to C terminal amino acids 175-194 of Human Glutathione Peroxidase
Run BLAST with
Run BLAST with
General notes
ab21966 reacts with cellular glutathione peroxidase. This is a 201 amino acid selenium
dependant cytoplasmic enzyme that exists as a homotetramer. This enzyme protects red blood
cells and other cells from oxidative damage.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Purification notes
0.2 ml filtered lyophilised serum.
Primary antibody notes
ab21966 reacts with cellular glutathione peroxidase. This is a 201 amino acid selenium
dependant cytoplasmic enzyme that exists as a homotetramer. This enzyme protects red blood
cells and other cells from oxidative damage.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab21966 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Dot Blot
1/500000.
1/500,000 on 500 ng of antigen.
IHC-P
1/1000 - 1/20000.
Dilution depending on tissue and detection method. Antigen retrieval has been
used in characterisation but may not be necessary.
IHC-Fr
1/1000 - 1/20000.
Dilution depending on tissue and detection method.
WB
1/10000 - 1/100000. Predicted molecular weight: 22 kDa.
Dilution depending on detection method.
ELISA
1/2000 - 1/20000.
Target
Function
Protects the hemoglobin in erythrocytes from oxidative breakdown.
Sequence similarities
Belongs to the glutathione peroxidase family.
Cellular localization
Cytoplasm.
Anti-Glutathione Peroxidase 1 antibody images
DAB staining of microglia with GPx1S in
human brain tissue. Primary antibody (1/400)
secondary antibody donkey anti-sheep biotin (1/3000) followed by Vector ABC kit.
Immunohistochemistry - Glutathione Peroxidase
antibody (ab21966)
2
DAB staining of microglia with GPx1S in
human brain tissue. Primary antibody
(1/1000) secondary antibody donkey antisheep - biotin (1/3000) followed by Vector
ABC kit. (Oil emersion).
Immunohistochemistry - Glutathione Peroxidase
antibody (ab21966)
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Replacement or refund for products not performing as stated on the datasheet
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Terms and conditions
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