Anti-Glucose 6 Phosphate Dehydrogenase antibody ab106810

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Overview

Product name

Description

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

Anti-Glucose 6 Phosphate Dehydrogenase antibody

Goat polyclonal to Glucose 6 Phosphate Dehydrogenase ab106810 is expected to recognise isoforms NP_000393.4 and NP_001035810.1.

WB, IHC-P, Sandwich ELISA

Reacts with: Human

Predicted to work with: Rat, Sheep, Dog

Synthetic peptide:

C-STNSDDVRDEKVK

, corresponding to internal sequence amino acids 308-320 of Human Glucose 6 Phosphate

Dehydrogenase (NP_000393.4).

Run BLAST with

Human Testis, Thyroid and Tonsil lysates; Human testis tissue.

Run BLAST with

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Isotype

Liquid

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Preservative: 0.02% Sodium Azide

Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Immunogen affinity purified ab106810 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Polyclonal

IgG

Applications

Our Abpromise guarantee covers the use of ab106810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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Application

WB

IHC-P

Sandwich ELISA

Abreviews Notes

Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 59 kDa.Can

be blocked with Glucose 6 Phosphate Dehydrogenase peptide (ab196304) .

Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use a concentration of 5 - 10 µg/ml. Increasing amount of recombinant G6PD captured by a rabbit antibody. ab106810 used as reporter antibody.

Target

Anti-Glucose 6 Phosphate Dehydrogenase antibody images

Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg developed using the ECL technique

Predicted band size : 59 kDa

Western blot - Glucose 6 Phosphate

Dehydrogenase antibody (ab106810) ab106810, at 2.5 µg/ml, staining Glucose 6 phosphate dehydrogenase in paraffinembedded Human testis tissue by

Immunohistochemistry using AP staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6

Phosphate Dehydrogenase antibody (ab106810)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Our Abpromise to you: Quality guaranteed and expert technical support

Replacement or refund for products not performing as stated on the datasheet

Valid for 12 months from date of delivery

Response to your inquiry within 24 hours

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