Product datasheet
Anti-COX2 / Cyclooxygenase 2 antibody ab6665
10 References
Overview
Product name
Anti-COX2 / Cyclooxygenase 2 antibody
Description
Rabbit polyclonal to COX2 / Cyclooxygenase 2
Specificity
The antibody does not cross react with Cox-1.
Tested applications
WB, ELISA, Dot Blot
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fusion protein (Human C-terminal end)
Epitope
carboxy-terminus of human Cox-2.
General notes
COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its
discovery was followed by a period of discovery and drug development to create a “super
aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of
brain and kidney function in search of better treatments for degenerative and inflammatory
diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab6665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ELISA
Dot Blot
1
Application notes
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in
response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and
transient expression.
This antiserum against Human Cox-2 and has been tested for use in immunoblotting (1:5,000 to
1:10,000) and ELISA (1:10,000 to 1:50,000).
The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in
WISH cells induced with IL-1b.
Target
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major
mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
Pathway
Lipid metabolism; prostaglandin biosynthesis.
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Post-translational
modifications
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on
different Cys residues in addition to Cys-561.
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
2