PACKING SLIP
THE MIDLAND
CERTIFIED REAGENT COMPANY
3112-A West Cuthbert Avenue
MIDLAND, TEXAS 79701
Customer Number: NGUYEN Q
Customer Name: QUOL THANG NGUYEN
PO Number:
Terms:
Date:
(800) 247-8766 Ext
Bill To: QUOL THANG NGUYEN
UNIVERSITY OF CA-SAN DIEGO
DISBURSEMENTS DIVISION 0955
9500 OILMAN DRIVE/ PO#20326798
LAJOLLACA 92093-0955
Oligo Name
Oligo
Number
Length
Grade
20326798
NET 30
9/21/05
Ship To:
Scale
QUOL THANG NGUYEN
QUOL THANG NGUYEN
UREY HALL ROOM 7108 MC0319
9500 OILMAN DRIVE
SAN DIEGO CA 92093
Lot Number
Order #
1
37mer RP
1
092605-00148
OPTS00031777
5 ' - ( TXRED)( C6Amino)TAGGGATCCTCTGTGGCCCAATTTGCAACCAGCCCTA( BHQ-2)-3'
TARGET
2
27mer
GF
.05
291905-0004C
5 ' -CTGGTTG(IAAATTGGGCCACAGAGGAT-3 '
Thank You!
Not For Drug Use, For Research Only
OPTS00031777
THE M I D L A N D CERTIFIED REAGENT C O M P A N Y , INC,
3112-A West Cuthbert Ave. • Midland, TX 79701 • Voice: 800-247-8766 or 915-694-7950
Fax: 915-694-2387 • e-mail: robb^if-oiimto-com • Web site: iiiip://\v\\w.iiicrc.coni
Molecular Beacon Analysis
The Molecular Beacon (Lot 092605-00148; 5' TXRED- TAGGG
ATCCTCTGTGGCCCAATTTGCAACCAG CCCTA -BHQ2 3^) and target (Lot
291905-0004C;
5'-CTGGTTGCAAATTGGGCCACAGAGGAT -3') were analyzed in our laboratories to
determine the signal-to-noise ratio ofthis pair.
The analysis was performed at RT on a Hitachi F-2000 Fluorescence
Spectrophotometer, utilizing a 2 ml cuvette and a buffer containing 20 mM Tris-HCl, pH
8.0, 50 mM KCI, and 5 mM MgC^. The excitation wavelength used was 583 nm and the
emission wavelength was set to 603 nm. Two milliliters of buffer was added to the
cuvette and the arbitrary fluorescence units were noted for the buffer alone (Fbufter)Molecular Beacon was added to the cuvette to a final concentration of 50 nM. The
increase in fluorescence due to background was allowed to stabilize and the value was
recorded (Fdose)- A 4-fold molar excess ofthe target solution was added and the
fluorescence was monitored for a total of 2970 sees. The highest value was recorded
11^ open/-
The Signal to background to noise ratio was calculated as follows:
(Fopen - Fluifrcr)/(Fclose " Fhuller)
'bufTe
open
S/N
0.0185
0.317
4.228
14.1
Documented b
This Molecular Beacon Probe is sold under a license to The Midland Certified Reagent Company, granted
by the Public Health Research Institute ofthe (4ty of New York, Inc. This product comes with
only limited field-of-use rights for research puiposes only, under PHRl Patent Rights.
'^)k
THE MIDLAND CERTIFIED REAGENT COMPANY INC.
(g^tttititatc of ^nalpsf;^
No.
-GP
The product described below was manufactured in ttie laboratories of The IVIidland Certified
Reagent Company Inc. of Midland, Texas.
The analytical data reported hereon were obtained in the laboratories of The Midland Certified
Reagent Company Inc. by individuals qualified to perform the analytical procedures. Details of
analytical procedures are available to bona fide customers free of charge on request.
PRODUCT.
LOT NUMBER.
291905-0004C
This custom oligodeoxynucleotide was synthesized using cyanoethy! phosphoramidite
chemistry. After removal ofthe protecting groups by hydrolysis with concentrated
ammonium hydroxide, the product was desalted by gel filtration chromatography (hence GF
grade). GF grade oligonucleotides are generally suitable for use as sequencing primers,
primers for amplification of DNA or RNA, and for many other purposes. The oligonucleotide
has been dried in the ammonium salt form, althought trace amounts of otherions may also be
present. The quantity of material is given in nanomoles and in A260 Units. One A260 Unit is
equivalent to approximately 30-35 micrograms of DNA. The molecular weight (MW) is
calculated for the free acid form ofthe DNA.
Order #:OPTS00031777
Name: TARGET, 27-mer
Ordered By QUOL THANG NGUYEN
Sequence:
5'-CTGGTTGCAAATTGGGCCACAGAGGAT-3'
Mw(g/mol) = 8364.7Tm61.2
Number ot shipping Vials: 1
Quantity:
Hg/vial =132.4
nmole/vial =15.8
A?,fin / vial =4.7
W^:-
Released 1
Richard V. Case, Ph.D.
W^i
m
THE MIDLAND CERTIFIED REAGENT COMPANY INC.
,£^tttvtitatt of ^mipsi;^
No.
RP
The product described below was manufactured in the laboratories of The Midland Certified
Reagent Company Inc. of Midland, Texas.
The analytical data reported hereon were obtained in the laboratories of The Midland Certified
Reagent Company Inc. by individuals qualified to perform the analytical procedures. Details of
analytical procedures are available to bona fide customers free of charge on request.
PRODUCT
LOT NUMBER.
092605-00148
i
This custom oligodeoxynucleotide was synthesized using cyanoethyl phosphoramidite
chemistry. After removal ofthe protecting groups by hydrolysis with concentrated ammonium
hydroxide, the product was purified by Reversed Phase HPLC (RP grade). A copy ofthe HPLC
elution profile is attached to this Certificate of Analysis. With the exception of
oligonucleotides containing 5' Thiol Modifier C6, the trityl-positive fractions were pooled,
dried, and detritylated, The oligonucleotide was purified from the frityl group by gel
filtration chromatography. The product is ftimished as the dried ammonium salt form unless
otherwise indicated on theOligonucleotide Synthesis Record.The quantity of material is given
in nanomoles and in A260 Unit s. One unit is equivalent to approximately 30-35 micrograms o
DNA. The molecular weight (MW) is calculated for the free acid form ofthe DNA.
es^
Order #: OPTS00031777
Name:, 37-mer
Ordered By QUOL THANG NGUYEN
Sequence:
5'-(_TXRED)(_C6Amino)TAGGGATCCTCTGTGGCCCAATTTGCAACCAGCCCTA
Q-2)-3'
Mw(g/mol)= 12742.1Tm69.6
Number ot shipping Vials: 6
Quantity:
Hg / vial =224.5
nmole/vial =17.6
''M
Released By:
i-'^^jS^s
CUSTOM OLIGONUCLEOTIDE SYNTHESIS RECORD
Operator
A ' 2 ^ '^
«i : 75"<^ 5 6 7 8
9 10 1112
6 H Date "t /?(// t f Time
Lot #_0^2^O5HHi
Customer Quoi T)^A»^ Kl^uyi"*^
13 14 15 16 Oligo Name
17 1^<|)20
Verified by
PHOSPHORAMIDITES:
SUPPORT: A C G T 1 U NH, V-%^-Z
LL 500A lOOOA
Mfgr_^|£)ni__ Lot # &Si>l-2Zii>
PURGE?
Net Weight
grams
A //,( to
@
c
^mole/g
G/Z.l to
@
c
C ff,r to
@
c
prepared by 6H
TJOAJO
_@_
ORDER INSTRUCTIONS
X= Am]r.r> Of TFA Lot # Q g ^ W T g base J1 ^ ](^^
,OZ78^
X Loading
^0
JJL
jimole
- ^
NO by ^f)
DNA Grade: GF AE PAGE T S P ( ^ X Port Date
Tfi^ON
^-^7-6
Service: ND STD AE PAGE TSP
REAGENTS:
Ship: 1 2 4 10 & ALL_
WashACN
ivo
/
Tetrazole
go
/
Capping A
^0
Capping B
^Q
Ship: M Tu W Th F S
OD
/_
DEPROTECTION:
5 Vial
Vial ##
(g),
^ ' ^ °C
V | t i ^ ^ _ ^ i ^ _ j g ) p by
/ /
Hiush
/(3 ^ P by
TRITYLS
,
Total #=
^00^
Oxidizer
^0
DEBLOCKING
COLUMN
Date:£2l^Mi^
Tube
Abs a r > v
No. 52553^(545)
Final
Detritylating
PA
C
/
DESALTING COLUMN
Absorbance at 260 nm
Date: loejof
Time: l(;o S^/r—
un by
2
ube#
3
1
2
3
4
5
by.
#
^
6
Read by
rV
//L
Packaged b;
rev. 5/24/95
POST SYSNTIISIS LABELING LOG
Tech initials
P^
Oligo Lot U:
Dye
/^Jxiof^^S^
d^4fM^M-
Manufacturer
Labeling pH ^ > ' ^
%iXl*^r-
Date: ^ 1 ^ o < "
Time: S^'KtJ
Scale
^3'/Inlemal
/1^
Fomi: h | l ^ / ITC / Other
Dye Lot U: M T l l O (
Product tfr^£>03^
Buffer: (^aCOa/NaHC^O]^ Other Q>(^
Recovery procedure: Precipitation / B ^ c o l u ! ^
092605-148.BOO
3.0
:2
i0
12
il
2i22
i'^
2\
6'
3.0
2"""T^E
•• : D
2.5
2.5
2.0-
2.0
i
!l
1.5
1.5
1.0
1.0
0.5
0.5
0.0
•-'- —'
-0.0
0
Min
10
2
6
0
n
m
Date 10/05/85
Ratio/Equation Report
Time 15:42:24 Page 1 of 1
Method file : <untitled>
Information : Default Method
Data File
: <untitled>
Overlai d Spectra:
0.4-
/'
/
0.35 H
0.3- /
i.
0.25^
i °'^^
1
<
0.15 r
\\,
V
0.1 H
'-•••"~1—
.
^
>
^
.^_^^
v_
0.05-
—-.-^-^-^.^ .^_-
0^
1
250
.'
• > •
300
350
400
450
500
550
-('^w-
\
^ ^ ^ ^- %- _^i ; ^
Wavelength (nm;
Equation : Ratio = WL3/WL2
Where
: WLl = Abs(260nm), WL2 = Abs(579nm), WL3 = Abs(592nm), Wt =
Weight, V = Volume
#
Name
Dilut. Factor
Ratio
Abs<260nm>
1.00000
1.00000
1.11800
1.14720
0.28704
0.38692
1
2
#
Name
1
2
Report generated by
Abs<57 9nm>
Abs<592nm>
8.5186E-2
0.11919
9.5234E-2
0.13673
ROBE
Signature;
*** End Ratio/Equation Report ***
Tll^F,
^
SCAN/n926-148
10/05/05
sec
0
30
60
90
120
150
180
210
240
270
300
330
360
390
420
450
480
510
540
570
600
630
660
690
720
750
780
810
840
870
900
930
960
990
1020
1050
1080
1 1 10
1 140
1 170
1200
1230
1260
1290
1320
1350
1380
1410
1440
1470
1500
1530
1560
1 590
1620
1650
1680
1710
1740
1770
1800
1830
I860
1890
1920
1950
1980
20 1 0
2040
207n
0.0 18)(;.wff<'^
0. oigy
^^il-fo^
1 . 663
2. 222
2. 429
2. 534
2.606
2. 654
2. 683
2. 700
2.726
2. 750
2. 759
2. 776
2. 7 8 2
2.809
2. 822
2. 838
2.837
2.856
2.867
((J
^
^
2.867 A J
2 . 890 i'"^
2. 906
2, 933
2.920
2,918
2. 937
2. 955
2. 953
2. 979
2. 977
2. 986
2.981
3.009
3.006
2. 962
2. 942
2. 969
3,014
3. 159
3.326
3.517
3,692
3,829
3,954
4, 001
4, 068
4.089
4, 105
4,117
4, 130
4.141
4.111
4. 132
4. 135
4. 128
4. 124
4.114
4. 129
4.145
4. 1 55
4. 183
4. 189
4, 188
4. 180
4. 1 82
4. 179
-n . 1 a/1
15:47
2100
2130
2160
2190
2220
2250
2280
2310
2340
2370
2400
2430
2460
2490
2520
2550
2580
2610
2640
2670
2700
2730
2760
2790
2820
2850
2880
2910
2940
2970
TIMK
4. 198
4. 187
4, 203
4. 21 5
4. 228''(c^t/^
4. 187
4. 207
4. 193
4, 184
4, 193
4. 206
4. 198
4, 204
4. 207
4. 203
4. 197
4. 198
4, 208
4. 206
4, 218
4.214
4, 206
4. 204
4. 209
4, 218
4, 230
4, 216
4,222
4.215
4, 216
SCAN/0926-148
l^-J
0/05/05
16:38
Midland Certified Reagent Co.
Method: STD-DNA1
Accelerating Voltage: 20000
Grid Voltage: 92.000 %
Guide Wire Voltage: 0.050 %
Delay: 375 ON
Sample: 55
Original Filename: h:\masspec1\092605\00148-02.ms
This File # 3 = H:\MASSPEC1\092605\00148-02.MS
Comment: See file name for lot #
Laser: 2400
Scans Averaged: 64
Pressure: 1.13e-06
Low Mass Gate: 700.0
Negative Ions: ON
Collected: 10/5/05 5:20 PM
7000
6000
5000
4000
3000
2000
6000
7000
8000
9000
10000
Mass (m/z)
11000
12^00
13000
14000