Biomedical E ngineer ing
Protein Secretion
•  ELISA
•  TGFBeta1, TIMP1, MMP
•  Key findings
1.  TGFBeta 1 and MMP
production is sensitive to
media type.
2.  Not affected by impact number
(single versus double)
3.  Mixed sensitivity to impact
type.
Biomedical E ngineer ing
Protein Secretion
•  ELISA
•  TGFBeta1, TIMP1, MMP
•  Key findings
1.  TGFBeta 1 and MMP
production is sensitive to
media type.
2.  Not affected by impact number
(single versus double)
3.  Mixed sensitivity to impact
type.
4.  TIMP1/MMP2 ratio increased
following impact (serum)
Biomedical E ngineer ing
Conclusions
•  Astrocytes are mechano-sensitive
•  ECM protein gene expression is
reduced in response to impact
•  Impact number plays a key role in
the astrocyte response
Cell seeded substrate
•  Astrocytes were sensitive to both
deceleration only as well as
combined impact.
What Next
•  Exploring neuronal interactions with
ECM (neuro-inhibitory?)
ECM collected from substrate
UAMS
Shahryar Ahmadi MD
Biomedical E ngineer ing
Fort Sam Houston
Tom Walters PhD
UAF
Tyrone Washington PhD
Kartik Balachandran PhD
Shiloh Hurd
Ben Kasukons
Zach Heller
Joe Wyatt
John Kim
Addison Walker
Klaire Wilson
#CMMI-1404716
R15AR064481