MEDICAL MICROBIOLOGY (I) (MLT-202)
2nd YEAR, 2nd SEMESTER - PRACTICAL
UNITS
1
CONTENTS
Safety Rules and General Instructions
HOURS
2
Use and Preparation of Selective
and Differential
Media
- Mannitole salt agar
- Blood agar
- Chocolate agar
2
- Sabouraud agar
- MacConckey agar
- Muller Hinton agar
- Eosin methylene blue agar
- Nutrient agar
Collection and Transport of Clinical
Samples and Normal Body Flora
- Skin
51
- Ears
- Upper respiratory tract (nasal and throat
swab)
3
4
- Lower respiratory tract (Sputum)
- Alimintary tract (faeces)
- Urinary tract (Urine)
Bacterial Staining.
- Simple staining
4
- Gram Staining- Acid-fast Stain
- Spore Stain
- Capsule Stain
Biochemical Activities of Microorganisms
- Extracellular enzymatic activity
- Carbohydrate fermentations including
glucose, sucrose broth and triple sugar iron
51
1
agar.
21
- IMVIC test
- Hydrogen sulfide-indole motility test (SIM
agar)
- Enzymes like catalase, oxidase and urease)
- Litmus milk reduction
Genus and Species Identification
of
Unknown Bacteria
- API test
6
4
- Unknown bacterial isolate is to be identified
by students and results are provided in a
report at the end of the semester
Antimicrobial Tests
- Kirby-Bauer antimicrobial susceptibility
test.
7
- Synergistic effect of drug combinations
REFERENCES
- Medical Microbiology: A Guide to Microbial Infections
:Pathogenesis, Immunity, Laboratory Diagnosis, and Control By
1
David Greenwood, Michael R. Barer, John F. Peutherer, Richard C.
B. Slack , Edition: 17, Published by Churchill Livingstone, 2007
- Practical Handbook of Microbiology By Emanuel Goldman, Lorrence
H. Green, 2nd edition, Published by CRC Press, 2008