Supplementary Information:
Supplementary Text
Supplementary References
Figure S1
Figure S2
Figure S3
Figure S4
Figure S5
Figure S6
Table S1
Table S2
Table S3
Supplementary Text:
The frequency of gene coding DNA (cDNA) migration in relationship to
geographic distance is shown on a plot of sample site GPS coordinates within YNP
(Figure 1). YNP regions from Figure 1 are depicted in Figures S1 through S4, focusing in
on several well sampled regions within the Park, including Lower Geyser Basin (Figure
S1), Bison Pool (Figure S2), Norris Geyser Basin (Figure S3) and Mammoth Hot Springs
(Figure S4). As with Figure 1, in Figures S1 through S4 sites are connected with lines
demonstrating recent cDNA migration with line thickness indicates normalized migration
quantity and thicker lines indicating higher quantities of DNA migration/sharing between
sampled sites. Sites and connections are colored as phototrophic (blue) and chemotrophic
(red), with three examples of chemotrophic to phototrophic cDNA migration in green (all
three involve BP3, a ‘transitional’ chemotrophic community positioned just above the
photosynthetic fringe in Bison Pool. Figures S1 through S4 are broken into four
quadrants of increasing Ks ranges (Ks < 0.01 at top left, 0.01 <= Ks < 0.07 at top right,
0.07 <= Ks < 0.32 at bottom left and 0.32 <= Ks < 0.50 at bottom right. As reiterated in
iterative multiple linear regression analysis (in main text), figures S1-S4 underscore that
scaffolds are shared/migration can occur over long distances across YNP; geographic
distance is not a primary factor in determining the frequency of DNA migration between
sites. For instance, there is an absence of DNA migration between YNP 7 and
neighboring sites (including YNP 3, 8, 14, and 19) despite these sites lying only a few
kilometers apart, whereas DNA migration is occurring over 70+ km separating YNP 12
and YNP 13. Migration most commonly occurs between sites whose communities
employ similar metabolic strategies, i.e. shared scaffolds are detected between
phototrophic sites and chemotrophic sites, but seldom between the two. BP 3, mentioned
above, is a noted exception, with low Ks scaffolds (arguably recent migrations) shared
with BP 4, BP 5 and YNP 15 (all phototrophic sites. The most abundant DNA migrations
occur between Aquificae-dominated alkaline environments, including YNP 11, YNP 12,
YNP 13, BP1 and BP2. Concomitantly, the range of environments that host Aquificae
span the entire geographic area of the Park. Additionally, strong links exist between
acidic sites, including YNP 4, YNP 8, YNP 9 and YNP 18, which also cross the width of
the Park. Interestingly, YNP 4 and YNP 12 are similar temperature (78oC vs 75oC,
respectively) chemotrophic sites with a relatively small geographic separation (~15 km),
yet these two communities show no shared DNA fragments—despite both being DNA
migration ‘hubs’ to other communities, each sharing fragments with multiple other sites
across the Park. This reiterates the idea that multiple environmental factors are involved
in selecting for, maintaining, and also attenuating the biological connections between
sites.
Within the plots shown in Figures S1 through S4, the majority of DNA migrations
in the lowest Ks quadrant (top left) are among phototrophic sites (blue lines) with
migrations among chemotrophic sites (red lines) becoming prevalent in mid-Ks ranges
(top right and lower left), until the highest Ks quadrant (lower right) is dominated by
migrations among chemotrophic sites (red lines). These observations in essence represent
the overall trends shown Figure 3 in the main text: phototroph communities appear to be
sharing DNA more frequently across separate sites. Intriguingly, several shared scaffolds
were detected between YNP and GBS (a distance of ~800km), though these are present
only in the highest Ks quadrant, consistent with the large temporal and
geological/geographical separation between GBS and YNP communities.
Principal components analysis (PCA) was performed on 20 of the geochemical
parameters measured across all YNP metagenomic sites (Figure S5A). This analysis
resulted in two principal components (PC1 and PC2) accounting for 44.2% of the
geochemical variation between sampled locations. A biplot of PC1 verses PC2 (Figure
S5A) indicates the location of metagenomic sites in geochemical space with vectors
indicating the directionality and magnitude of influence of each geochemical parameter.
Sampled locations in similar regions of YNP are circled and tight clustering of
geochemically and geographically distinct regions within YNP is clearly visible. The five
samples taken along the Bison Pool outflow channel (within the Lower Geyser Basin
grouping) demonstrate tight clustering despite a 32oC temperature gradient, confirming
the PCA analysis is clustering across multiple geochemical dimensions that, for Bison
Pool in particular, remain relatively constant along the outflow channel. Additionally, pH
is a major vector along PC2, separating the acidic Norris Geyser Basin sites from the
alkaline Lower Geyser Basin sites.
Figure S5B maps DNA migration in this PCA space, using lines to represent
DNA migration between sites sorted based on their physico-chemical similarities. Line
thickness is proportional to the number of shared scaffolds between sites and lines are
color-coded to highlight community metabolism, as described above. Notabl, proximal
points in this PCA space tend to have the strongest connections; shared scaffolds are most
frequently found between communities that occupy geochemically similar environments.
This is especially evident with the three phototrophic-to-chemotrophic connections, all
occupying a very small region of the PCA plot. Despite the geographic separation of ~10
km between BP3 and YNP 15, they are closer than BP3 and BP5 in geochemical space
(~10m separation), further supporting the notion that “environment selects” what
migrations are ultimately fixed in a community. YNP 4, a slightly acidic site with pH
6.1, is serving as a bridge with connections to both acidic sites (YNP 8 and YNP 14) and
alkaline sites (YNP 11 and BP1). The Mammoth Hot Springs area is poorly connected to
the rest of YNP, as is demonstrated by YNP 10 and YNP 18 exhibiting only weak
connections despite being geochemically similar chemotrophic sites. While distance is
frequently overshadowed by other factors in predicting numbers of shared scaffolds
between sites, hydrothermal regions within the Park do appear in this PCA space (circled
areas in Figure S5), and the hot springs within these regions do show broad geochemical
similarities (Table S1). However, YNP 13 is ~25 km away from BP1 and yet YNP 13 and
BP1 demonstrate the largest quantity of high identity scaffolds shared between any pair
of sites across the Park. The PCA analysis also accounts for the lack of migration
between YNP 4 and YNP 18, which are less than 10 km apart but reside on opposite sides
of the PCA plot, with YNP 4 clustering near the Lower Geyser Basin sites and YNP 18
plotting closer to the Mammoth Hot Springs sites in Figure S5.
To investigate biases in the phyla responsible for DNA migration between sites,
2-way clustering was done using all scaffolds in each metagenomic site (Figure 2) and
the scaffolds involved in DNA migration (Figure S7B). Phyla were assigned as the best
hit within the NCBI nt database, if one existed, for all scaffolds over 3,000bp in length.
The taxonomic family of all cDNA fragments involved in DNA migration was
determined to investigate bias in Ks distribution and migration frequency. Figure 2
contains a bar plot which demonstrates the fraction of all cDNA migrations assigned to
each taxonomic family, including the fraction of unclassified cDNA sequences
transferred between chemotrophic (top) and phototrophic (bottom) sites. Families are
ordered and colored by their distribution within chemotrophic (top - red) and
phototrophic (bottom - blue) sites, with families found within both phototrophic and
chemotrophic communities colored grey. Additionally, a Ks histogram was generated for
each taxonomic family between Ks values of 0.00 and 0.50 (right of family name). The
largest percentage of cDNA migrations are attributed to unclassified cDNAs from
chemotrophic sites (25%), with Aquificacaea (25%) and Chloroflexaceae (10%)
accounting for the highest percentages of chemotrophic and phototrophic transfers.
Interestingly, none of the families commonly found within both chemotrophic and
phototrophic sites (grey bars) accounted for more than 1% of cDNA transfers, indicating
a strong bias towards the migration of families limited to either high temperature
(chemotrophic) sites or photosynthetic families. The Ks histograms for highly transferred
families mirror the overall trends seen in Figure 1, for instance with the Aquificacaea
histogram representing a Poisson distribution centered at 0.18 and, similarity,
Acidobacteriacea, Chloroflexaceae and Synococcacaea (all known photosynthetic phyla)
demonstrating large peaks at very small (<0.01) Ks values.
Supplementary References:
35.
I. V. Grigoriev et al., The Genome Portal of the Department of Energy Joint
Genome Institute, Nucleic Acids Res. (2011), doi:10.1093/nar/gkr947.
36.
S. F. Altschul, W. Gish, W. Miller, E. W. Myers, D. J. Lipman, Basic local
alignment search tool, J. Mol. Biol. 215, 403–410 (1990).
37.
M. Kanehisa, S. Goto, KEGG: Kyoto Encyclopedia of Genes and Genomes,
Nucleic Acids Res. 28, 27–30 (2000).
38.
Z. Zhang et al., KaKs_Calculator: calculating Ka and Ks through model selection
and model averaging, Genomics Proteomics Bioinformatics 4, 259–263 (2006).
Figure S1:
Figure S2:
Figure S3:
Figure S4:
Figure S5:
Table S1: YNP environmental metadata for 24 YNP metagenomes used in this study, listed by sample site shorthand and hot spring
name (bdl=concentrations below detection limits, mM=millimolar aqueous concentrations, ionization/speciation given if known)
T
pH
°C
Na+
mM
K+
mM
Al
mM
Fe
mM
Mg
mM
ClmM
NH4+
SO4-2
NO3-
mM
mM
mM
mM
P
mM
Si
mM
B
mM
As
mM
Zn
mM
Mn
mM
S2mM
O2
mM
425
0.04
685
8.74
bdl
5.2
5.36
0.11
2.0
3.1
10.2
bdl
bdl
Ca+2
Alice Springs
75
2.1
1.1
0.38
0.68
396
227
YNP2
Nymph Lake
89
4.7
4.6
0.29
0.39
37.4
1.5
57
4.1
230
1.34
5.8
0.11
2.82
0.26
8.7
1.3
33.9
3
bdl
YNP3
Monarch Geyser
85
4
9.5
0.64
0.08
29.6
3.6
8.6
9.7
277
1.33
12
bdl
5.29
0.58
19.4
1.4
1.3
7.8
bdl
YNP4
Joseph's Coat
78
6
10.5
2.00
0.42
3.0
1.6
43.6
8.9
8424
3.79
12
bdl
5.58
5.85
165
bdl
5.3
20
bdl
YNP5
Bath Lake
57
6.2
3.93
0.97
8.82
bdl
0.7
2132
4.4
40
5.60
4.8
87.7
0.66
0.31
24
0.3
0.2
117
bdl
YNP6
White Creek
52
8.2
3.59
0.39
0.42
3.3
1.7
13.8
1.8
1.9
0.23
2.1
3.2
3.83
0.07
5
0.2
4.7
bdl
188
YNP7
52
5.8
4.12
0.44
0.47
2.7
75.5
58.8
0.89
4.2
0.23
2.2
54.9
1.38
0.04
9
bdl
24
bdl
bdl
73
3.3
10.8
0.28
0.11
64.8
36.6
14
14.2
88
1.05
19
bdl
10.4
0.69
28.4
1.5
1.6
10
22
YNP10
Chocolate Pots
One Hundred Springs
Plain
Narrow Gauge
71
6.5
4.42
1.10
8.40
0.7
0.4
2219
4.55
40.1
5.66
6.5
38
0.72
0.30
20
0.0
0.4
67
bdl
YNP11
Octopus Spring
82
7.9
12
0.34
0.01
8.1
0.2
bdl
6.60
2.3
0.14
6.0
9
6.11
0.24
27.0
0.0
bdl
0
34
YNP12
Calcite Spring
75
7.8
8.81
2.40
0.60
8.1
3.2
251
6.62
1660
3.41
9.4
bdl
3.40
3.09
21
0.2
1
105
bdl
YNP13
81
7.8
9.70
0.34
0.10
1.7
bdl
1
0.22
bdl
bdl
2.90
bdl
2
0.3
1
bdl
bdl
73
3.5
10.8
1.38
0.10
65.3
31.6
9.1
87
1.18
18.5
bdl
5.67
0.65
24
1.0
1.2
3
30
59.9
8.2
12.6
0.64
0.02
2.9
0.1
0.8
2.00
13.2
0
7.30
bdl
YNP15
Bechler
One Hundred Springs
Plain
Mushroom Spring
4.4
0.18
6.6
bdl
4.51
0.25
26
0.5
0.1
bdl
141
YNP16
Fairy Geyser
35
8.1
9.38
0.10
0.05
3.1
bdl
3.5
5.20
1.3
0.18
6.7
11.9
0.21
13
bdl
bdl
bdl
31
YNP17
Obsidian Pool Prime
56
5.8
12.4
0.85
0.13
9.6
3.1
24.7
8.40
5.9
bdl
2.38
10.0
0
0.60
8
0.5
1
bdl
60
46.0
23
1.82
0.55
3.9
0.0
bdl
157
bdl
bdl
bdl
6.70
0.77
18.8
6.4
bdl
16.2
bdl
YNP1
YNP8
YNP14
YNP18
Washburn Spring
76
6.4
1.2
0.28
0.59
bdl
bdl
451
0.04
YNP19
Cistern Spring
76
4.4
11.3
0.97
0.05
8.2
0.2
3
14
0.6
2.50
2396
0
296
16.6
7
0.74
YNP20
Bath Lake
54
6.2
5.05
1.25
9.75
6.7
0.7
2625
5.70
40
7.20
5.6
bdl
0.70
0.30
23
0.3
bdl
117
bdl
BP1
Bison Pool Site 1
93.3
7.35
12.9
0.26
0.02
19.2
bdl
2.72
6.11
bdl
0.21
50.0
3.07
4.58
0.22
11.8
0.05
bdl
4.77
5.4
BP2
Bison Pool Site 2
79.4
7.68
13.2
0.26
0.02
19.6
bdl
2.57
6.29
bdl
0.20
50.0
2.80
6.34
0.24
12.3
0.06
0.01
2.03
24
BP3
Bison Pool Site 3
67.5
7.92
13.7
0.28
0.02
15.3
0.02
1.11
6.48
bdl
0.20
35.7
bdl
6.65
0.26
14.9
bdl
bdl
3.12
28
BP4
Bison Pool Site 4
65.3
8.00
13.8
0.28
0.02
29.1
bdl
2.51
6.52
bdl
0.20
42.8
3.64
5.80
0.31
15.8
0.03
0.01
4.68
50
BP5
Bison Pool Site 5
57.1
8.26
13.8
0.28
0.02
19.1
0.02
bdl
6.60
bdl
0.19
42.8
1.57
5.80
0.26
13.4
0.01
bdl
2.18
87.5
Table S2: Multiple Regression Results
All Metadata
CoefficientStd. Error
Intercept
NO3
K
SO4
t value
p value
1133.37
349.39
3.24
0.002
13.32
6.53
2.04
0.04
-610.94
320.50
-1.91
0.06
-67.51
43.90
-1.54
0.13
Zn
-126.60
82.58
-1.53
0.13
pH
-156.78
106.25
-1.48
0.14
O2
-3.61
2.53
-1.43
0.16
T
-18.47
13.60
-1.36
0.18
As
-7.64
5.82
-1.31
0.19
B
259.51
199.81
1.30
0.20
Na
-58.87
47.39
-1.24
0.22
GPS
1.65
1.87
0.88
0.38
Si
64.65
80.41
0.80
0.42
Cl
34.86
52.85
0.66
0.51
S
2.64
4.35
0.61
0.55
Al
1.99
4.42
0.45
0.65
P
-2.14
5.59
-0.38
0.70
Ca
-70.58
248.99
-0.28
0.78
Mg
0.27
1.00
0.27
0.79
Fe
-1.54
7.24
-0.21
0.83
Mn
-2.66
16.26
-0.16
0.87
Adjusted R-squared: 0.04, p-value: 0.25
After Iterative Multiple Linear Regression (iMLR)
t value
p value
Intercept
CoefficientStd. Error
1073.46
281.32
3.82
0.0002
K
-468.00
190.21
-2.46
0.02
12.08
5.56
2.17
0.03
-159.90
78.23
-2.04
0.04
SO4
-48.26
24.95
-1.93
0.06
T
-19.28
12.34
-1.56
0.12
B
238.30
154.31
1.54
0.13
As
-7.48
4.89
-1.53
0.13
O2
-3.23
2.25
-1.44
0.15
NO3
pH
Adjusted R-squared: 0.10, p-value: 0.01
Table S3: Over/under-representation of enzymes in shared metagenome fragments (EC enzyme commission #; K# KEGG orthology #; bold+italics
p<.01; italics p<.05)
EC
Annotation
Observed
Total
75
Observed
Shared
Fragments
175
Expected
Shared
Fragments
49
log2
odds
-1.84
pvalue
0.0002
4.1.1.61
K01612 4-hydroxybenzoate decarboxylase
6.4.1.1
biotin carboxylase
371
930
254
-1.87
0.0002
1.8.2.1
sulfite dehydrogenase SoxC precursor
30
55
17
-1.69
0.0024
1.4.3.19
K03153 glycine oxidase
125
211
66
-1.68
0.0028
1.3.3.1
K00226 dihydroorotate oxidase
355
6
71
3.56
0.0037
3.6.3.25
K02045 sulfate transport system ATP-binding protein
721
13
143
3.46
0.0041
2.6.1.42
K00826 branched-chain amino acid aminotransferase
330
7
66
3.24
0.0076
3.6.3.30
K02010 iron(III) transport system ATP-binding protein
1133
24
226
3.24
0.0076
2.6.1.62
DapA aminotransferase
127
172
58
-1.57
0.0097
2.4.1.83
K00721 dolichol-phosphate mannosyltransferase
536
6
106
4.14
0.0103
1.8.1.9
K00384 thioredoxin reductase (NADPH)
530
12
106
3.14
0.0106
5.4.99.12
K03177 tRNA pseudouridine synthase B
989
10
195
4.29
0.013
6.3.4.14
340
430
150
-1.52
0.0141
238
6
48
3
0.0162
2.7.7.42
K11263 acetyl-/propionyl-CoA carboxylase, biotin
carboxylase, biotin carboxyl carrier protein
K02013 iron complex transport system ATP-binding
protein
putative glutamate-ammonia-ligase adenylyltransferase
143
171
61
-1.49
0.017
3.1.1.29
K01056 peptidyl-tRNA hydrolase, PTH1 family
313
8
63
2.98
0.0172
2.7.1.2
K00845 glucokinase
274
7
55
2.97
0.0177
2.4.1.227
194
220
81
-1.44
0.0225
292
8
59
2.88
0.0226
8
8
3
-1.42
0.0249
1.1.1.3
K02563 UDP-N-acetylglucosamine--N-acetylmuramyl(pentapeptide) pyrophosphoryl-undecaprenol Nacetylglucosamine transferase
K00548 5-methyltetrahydrofolate--homocysteine
methyltransferase
K03280 UDP-N-acetylglucosamine:(glucosyl)LPS
alpha-1,2-N-acetylglucosaminyltransferase
K00003 homoserine dehydrogenase
336
10
68
2.77
0.0298
6.4.1.3
K01966 propionyl-CoA carboxylase beta chain
268
8
54
2.75
0.0312
4.1.3.27
K01657 anthranilate synthase component I
689
21
139
2.73
0.0327
3.6.3.34
2.1.1.13
2.4.1.56
2.5.1.72
K03517 quinolinate synthase
229
7
46
2.72
0.0335
6.6.1.1
K03403 magnesium chelatase subunit H
456
14
92
2.72
0.0335
6.1.1.24
glutamyl- and glutaminyl-tRNA synthetase
78
79
31
-1.35
0.034
1.1.1.132
K00066 GDP-mannose 6-dehydrogenase
32
33
13
-1.34
0.0354
1.3.3.4
K00231 protoporphyrinogen oxidase
127
124
49
-1.34
0.0354
5.4.3.2
lysine 2,3-aminomutase
122
119
47
-1.34
0.0354
1.1.1.267
1-deoxy-D-xylulose 5-phosphate reductoisomerase
209
200
80
-1.32
0.0382
2.7.2.11
K00931 glutamate 5-kinase
173
161
65
-1.31
0.0397
3.1.13.1
K01147 exoribonuclease II
95
86
35
-1.3
0.0412
4.2.1.46
K01710 dTDP-glucose 4,6-dehydratase
633
21
128
2.61
0.0426
2.6.1.85
K03342 para-aminobenzoate synthetase / 4-amino-4deoxychorismate lyase
K01586 diaminopimelate decarboxylase
144
131
54
-1.28
0.0443
257
221
93
-1.25
0.049
253
9
51
2.5
0.0531
1.2.7.8
K00166 2-oxoisovalerate dehydrogenase E1 component,
alpha subunit
indolepyruvate oxidoreductase subunit IorB
251
9
51
2.5
0.0531
4.1.99.3
K01669 deoxyribodipyrimidine photo-lyase
361
300
129
-1.22
0.054
5.4.99.5
K01850 chorismate mutase
202
163
71
-1.2
0.0574
3.1.1.61
K03412 two-component system, chemotaxis family,
response regulator CheB
K10206 LL-diaminopimelate aminotransferase
624
23
126
2.45
0.0583
223
179
79
-1.18
0.061
K06044 (1->4)-alpha-D-glucan 1-alpha-Dglucosylmutase
K01626 3-deoxy-7-phosphoheptulonate synthase
159
125
55
-1.18
0.061
262
10
53
2.41
0.0627
K01703 3-isopropylmalate/(R)-2-methylmalate
dehydratase large subunit
K01537 Ca2+-transporting ATPase
617
475
213
-1.16
0.0646
413
16
84
2.39
0.065
233
9
47
2.38
0.0662
4.2.1.17
K00965 UDPglucose--hexose-1-phosphate
uridylyltransferase
K01692 enoyl-CoA hydratase
331
13
67
2.37
0.0673
2.4.1.182
K00748 lipid-A-disaccharide synthase
141
104
48
-1.12
0.072
1.1.1.91
K05882 aryl-alcohol dehydrogenase (NADP+)
147
6
30
2.32
0.0733
4.1.1.20
1.2.4.4
2.6.1.83
5.4.99.15
2.5.1.54
4.2.1.33
3.6.3.8
2.7.7.12
150
6
30
2.32
0.0733
2.7.8.13
K00996 undecaprenyl-phosphate galactose
phosphotransferase
phospho-N-acetylmuramoyl-pentapeptide-transferase
200
145
67
-1.11
0.0739
1.1.1.86
K00053 ketol-acid reductoisomerase
262
188
88
-1.1
0.0758
2.8.1.6
K01012 biotin synthetase
281
203
95
-1.1
0.0758
1.2.99.2
697
29
142
2.29
0.0771
3.5.2.6
K03519 carbon-monoxide dehydrogenase medium
subunit
K01467 beta-lactamase
142
6
29
2.27
0.0797
1.6.99.3
K03885 NADH dehydrogenase
253
178
84
-1.08
0.0798
6.3.2.8
K01924 UDP-N-acetylmuramate--alanine ligase
284
201
95
-1.08
0.0798
3.6.3.17
542
23
110
2.26
0.081
2.4.1.18
K02056 simple sugar transport system ATP-binding
protein
K00700 1,4-alpha-glucan branching enzyme
197
136
65
-1.07
0.0818
1.7.99.4
K00372 nitrate reductase catalytic subunit
187
8
38
2.25
0.0823
2.8.3.16
K07749 formyl-CoA transferase
184
8
38
2.25
0.0823
2.5.1.90
K02523 octaprenyl-diphosphate synthase
164
7
33
2.24
0.0836
3.1.26.12
134
92
44
-1.06
0.0838
4.2.1.51
fused ribonucleaseE: endoribonuclease/RNA-binding
protein/RNA degradosome binding protein
K04518 prephenate dehydratase
234
161
77
-1.06
0.0838
6.1.1.2
K01867 tryptophanyl-tRNA synthetase
424
286
139
-1.04
0.0878
6.2.1.5
succinyl-CoA synthetase subunit beta
660
444
216
-1.04
0.0878
3.13.1.1
K06118 UDP-sulfoquinovose synthase
69
47
23
-1.03
0.0899
3.2.1.1
K01176 alpha-amylase
202
9
41
2.19
0.0904
4.1.2.4
K01619 deoxyribose-phosphate aldolase
198
9
40
2.15
0.0961
1.1.1.22
K00012 UDPglucose 6-dehydrogenase
485
309
155
-1
0.0962
1.13.12.16
K00459 nitronate monooxygenase
99
64
32
-1
0.0962
3.2.1.35
K01197 hyaluronoglucosaminidase
13
8
4
-1
0.0962
6.1.1.16
K01883 cysteinyl-tRNA synthetase
480
311
155
-1
0.0962
1.1.1.49
K00036 glucose-6-phosphate 1-dehydrogenase
193
122
62
-0.98
0.1005
1.20.98.1
K08356 arsenite oxidase large subunit
116
73
37
-0.98
0.1005
2.7.8.6
4.2.1.3
K01681 aconitate hydratase 1
541
340
172
-0.98
0.1005
4.2.1.9
K01687 dihydroxy-acid dehydratase
356
219
112
-0.97
0.1027
6.1.1.15
K01881 prolyl-tRNA synthetase
504
312
159
-0.97
0.1027
1.2.1.12
K00134 glyceraldehyde 3-phosphate dehydrogenase
333
16
68
2.09
0.1049
6.2.1.30
K01912 phenylacetate-CoA ligase
168
8
34
2.09
0.1049
1.17.1.2
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
305
15
63
2.07
0.108
2.4.2.18
anthranilate phosphoribosyltransferase
242
12
50
2.06
0.1095
2.5.1.7
243
12
50
2.06
0.1095
4.99.1.1
K00790 UDP-N-acetylglucosamine 1carboxyvinyltransferase
K01772 ferrochelatase
144
7
29
2.05
0.1111
4.2.1.11
phosphopyruvate hydratase
451
269
141
-0.93
0.1115
2.4.1.119
163
8
33
2.04
0.1126
286
166
88
-0.92
0.1138
2.1.1.35
K07151 dolichyl-diphosphooligosaccharide--protein
glycosyltransferase
K02495 oxygen-independent coproporphyrinogen III
oxidase
K00557 tRNA (uracil-5-)-methyltransferase
83
47
25
-0.91
0.116
3.3.1.1
K01251 adenosylhomocysteinase
359
208
111
-0.91
0.116
2.3.1.47
8-amino-7-oxononanoate synthase
141
82
44
-0.9
0.1183
2.7.7.4
K00958 sulfate adenylyltransferase
215
11
44
2
0.119
3.1.11.6
K03602 exodeoxyribonuclease VII small subunit
116
6
24
2
0.119
1.2.1.41
gamma-glutamyl phosphate reductase
201
114
62
-0.88
0.1229
2.1.1.32
LOC100276957
146
83
45
-0.88
0.1229
2.5.1.6
K00789 S-adenosylmethionine synthetase
447
248
136
-0.87
0.1253
2.6.1.17
K14267 N-succinyldiaminopimelate aminotransferase
251
139
76
-0.87
0.1253
3.5.1.98
K06067 histone deacetylase 1/2
110
62
34
-0.87
0.1253
3.6.1.1
K01507 inorganic pyrophosphatase
513
27
105
1.96
0.1256
3.5.1.28
K01448 N-acetylmuramoyl-L-alanine amidase
151
8
31
1.95
0.1273
3.6.5.3
K02355 elongation factor EF-G
1308
708
394
-0.85
0.13
1.2.4.1
K00163 pyruvate dehydrogenase E1 component
462
25
95
1.93
0.1306
3.1.2.6
K01069 hydroxyacylglutathione hydrolase
183
10
38
1.93
0.1306
1.3.99.22
1.4.7.1
K00284 glutamate synthase (ferredoxin)
164
9
34
1.92
0.1323
2.7.1.71
K00891 shikimate kinase
163
9
34
1.92
0.1323
1.2.7.3
1006
55
207
1.91
0.1341
1.97.1.4
K00175 2-oxoglutarate ferredoxin oxidoreductase
subunit beta
K04069 pyruvate formate lyase activating enzyme
693
38
143
1.91
0.1341
6.2.1.12
K01904 4-coumarate--CoA ligase
31
16
9
-0.83
0.1348
3.2.1.23
K12308 beta-galactosidase
199
11
41
1.9
0.1358
6.1.1.10
K01874 methionyl-tRNA synthetase
498
262
148
-0.82
0.1372
6.4.1.4
127
67
38
-0.82
0.1372
126
7
26
1.89
0.1375
5.1.1.4
K01968 3-methylcrotonyl-CoA carboxylase alpha
subunit
K00658 2-oxoglutarate dehydrogenase E2 component
(dihydrolipoamide succinyltransferase)
K01777 proline racemase
27
14
8
-0.81
0.1396
1.2.7.5
K03738 aldehyde:ferredoxin oxidoreductase
548
31
113
1.87
0.1411
4.6.1.1
K01768 adenylate cyclase
245
14
51
1.87
0.1411
4.3.2.2
K01756 adenylosuccinate lyase
379
195
112
-0.8
0.142
6.4.1.2
714
370
212
-0.8
0.142
2.7.6.5
K01961 acetyl-CoA carboxylase, biotin carboxylase
subunit
K00951 GTP pyrophosphokinase
304
156
90
-0.79
0.1445
2.7.7.22
K00971 mannose-1-phosphate guanylyltransferase
164
85
49
-0.79
0.1445
1.3.1.74
K08070 2-alkenal reductase
989
499
291
-0.78
0.1469
2.5.1.77
FO synthase subunit 1
196
98
57
-0.78
0.1469
2.5.1.48
K01739 cystathionine gamma-synthase
153
9
32
1.83
0.1483
1.5.99.8
K00318 proline dehydrogenase
100
6
21
1.81
0.1519
2.1.2.3
335
164
97
-0.76
0.1519
2.3.3.9
bifunctional phosphoribosylaminoimidazolecarboxamide
formyltransferase/IMP cyclohydrolase
K01638 malate synthase
136
8
28
1.81
0.1519
3.1.21.3
K01154 type I restriction enzyme, S subunit
744
44
154
1.81
0.1519
3.5.4.10
K11176 IMP cyclohydrolase
334
164
97
-0.76
0.1519
3.6.1.11
K01524 exopolyphosphatase / guanosine-5'triphosphate,3'-diphosphate pyrophosphatase
K00963 UTP--glucose-1-phosphate uridylyltransferase
171
10
35
1.81
0.1519
306
150
89
-0.75
0.1544
2.3.1.61
2.7.7.9
3.4.24.64
K01412 mitochondrial processing peptidase
272
135
80
-0.75
0.1544
1.1.2.4
K00102 D-lactate dehydrogenase (cytochrome)
182
11
38
1.79
0.1557
2.3.1.12
K00627 pyruvate dehydrogenase E2 component
(dihydrolipoamide acetyltransferase)
K00363 nitrite reductase (NAD(P)H) small subunit
186
11
38
1.79
0.1557
83
40
24
-0.74
0.1569
195
95
57
-0.74
0.1569
1.9.3.1
K00620 glutamate N-acetyltransferase / amino-acid Nacetyltransferase
K02267 cytochrome c oxidase subunit VIb
789
48
164
1.77
0.1594
1.12.1.2
NAD-reducing hydrogenase, alpha subunit
237
111
68
-0.71
0.1646
5.4.3.8
K01845 glutamate-1-semialdehyde 2,1-aminomutase
399
185
114
-0.7
0.1671
4.2.1.24
K01698 porphobilinogen synthase
251
16
52
1.7
0.173
2.5.1.1
K00787 farnesyl diphosphate synthase
266
17
55
1.69
0.1749
4.2.3.1
K01733 threonine synthase
588
265
167
-0.67
0.1749
2.2.1.1
K00615 transketolase
628
41
131
1.68
0.1769
2.4.2.14
K00764 amidophosphoribosyltransferase
355
158
100
-0.66
0.1775
2.6.1.1
K00812 aspartate aminotransferase
497
221
140
-0.66
0.1775
2.7.2.4
K00928 aspartate kinase
363
162
103
-0.65
0.1801
3.4.21.26
K01322 prolyl oligopeptidase
89
6
19
1.66
0.1809
3.5.2.3
K01465 dihydroorotase
442
193
124
-0.64
0.1828
4.1.1.36
phosphopantothenoylcysteine decarboxylase
296
129
83
-0.64
0.1828
6.3.2.5
K01922 phosphopantothenate-cysteine ligase
295
129
83
-0.64
0.1828
5.3.1.5
K01805 xylose isomerase
72
31
20
-0.63
0.1854
1.1.3.15
K00104 glycolate oxidase
395
169
110
-0.62
0.1881
3.1.3.11
fructose 1,6-bisphosphatase II
440
189
123
-0.62
0.1881
1.5.1.12
K00294 1-pyrroline-5-carboxylate dehydrogenase
177
12
37
1.62
0.1891
2.7.9.2
K01007 pyruvate, water dikinase
398
27
83
1.62
0.1891
2.3.3.13
K01649 2-isopropylmalate synthase
629
267
175
-0.61
0.1908
3.5.4.25
K01497 GTP cyclohydrolase II
242
104
68
-0.61
0.1908
6.3.4.18
K01589 5-(carboxyamino)imidazole ribonucleotide
synthase
237
101
66
-0.61
0.1908
1.7.1.4
2.3.1.35
3.6.3.4
K01533 Cu2+-exporting ATPase
495
34
103
1.6
0.1933
6.3.5.5
K01955 carbamoyl-phosphate synthase large subunit
1041
437
289
-0.6
0.1935
2.6.1.11
359
151
100
-0.59
0.1961
1.1.1.271
K00821 acetylornithine/N-succinyldiaminopimelate
aminotransferase
K02377 GDP-L-fucose synthase
87
6
18
1.58
0.1975
2.7.11.1
serine/threonine kinase 35
1044
73
218
1.58
0.1975
3.6.1.19
K02428 nucleoside-triphosphate pyrophosphatase
172
12
36
1.58
0.1975
3.6.3.6
K01535 H+-transporting ATPase
87
6
18
1.58
0.1975
4.1.1.32
K01596 phosphoenolpyruvate carboxykinase (GTP)
145
10
30
1.58
0.1975
5.4.2.10
phosphoglucosamine mutase
88
6
18
1.58
0.1975
1.1.1.202
1,3 propanediol dehydrogenase
15
6
4
-0.58
0.1989
1.6.5.5
K00344 NADPH2:quinone reductase
209
85
57
-0.58
0.1989
4.1.1.17
K01581 ornithine decarboxylase
78
33
22
-0.58
0.1989
6.2.1.34
K12508 feruloyl-CoA synthase
16
6
4
-0.58
0.1989
1.4.3.16
K00278 L-aspartate oxidase
246
101
68
-0.57
0.2016
3.1.3.1
K01077 alkaline phosphatase
250
102
69
-0.56
0.2043
4.4.1.1
K01758 cystathionine gamma-lyase
78
31
21
-0.56
0.2043
3.1.3.5
stationary phase survival protein SurE
374
27
78
1.53
0.2082
2.1.2.2
K11175 phosphoribosylglycinamide formyltransferase 1
204
15
43
1.52
0.2103
2.7.7.24
K00973 glucose-1-phosphate thymidylyltransferase
504
37
106
1.52
0.2103
6.2.1.13
CoA-binding domain-containing protein
316
23
66
1.52
0.2103
2.7.7.13
K00966 mannose-1-phosphate guanylyltransferase
354
26
74
1.51
0.2125
3.6.3.21
K02028 polar amino acid transport system ATP-binding
protein
K01928 UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase
K01940 argininosuccinate synthase
287
21
60
1.51
0.2125
274
20
57
1.51
0.2125
333
131
91
-0.53
0.2125
K14469 acrylyl-CoA reductase (NADPH) / 3hydroxypropionyl-CoA dehydratase / 3hydroxypropionyl-CoA synthetase
K05903 NADH dehydrogenase (quinone)
82
6
17
1.5
0.2147
79
6
17
1.5
0.2147
6.3.2.13
6.3.4.5
1.3.1.84
1.6.99.5
3.4.21.53
K01338 ATP-dependent Lon protease
701
52
147
1.5
0.2147
6.2.1.1
K01895 acetyl-CoA synthetase
765
57
161
1.5
0.2147
1.18.6.1
K11333 chlorophyllide reductase iron protein subunit X
146
11
31
1.49
0.2169
2.8.1.7
K11717 cysteine desulfurase / selenocysteine lyase
537
208
146
-0.51
0.2181
6.1.1.21
K01892 histidyl-tRNA synthetase
401
155
109
-0.51
0.2181
3.2.1.21
K05349 beta-glucosidase
387
29
81
1.48
0.2191
3.4.11.9
K01262 Xaa-Pro aminopeptidase
240
18
50
1.47
0.2213
1.12.99.6
K06281 hydrogenase large subunit
197
15
41
1.45
0.2258
2.7.1.49
249
19
52
1.45
0.2258
3.6.3.3
K00941
hydroxymethylpyrimidine/phosphomethylpyrimidine
kinase
K01534 Cd2+/Zn2+-exporting ATPase
144
11
30
1.45
0.2258
2.7.1.17
K00854 xylulokinase
232
18
49
1.44
0.2281
4.1.1.37
K01599 uroporphyrinogen decarboxylase
242
90
65
-0.47
0.2292
4.1.99.12
K02858 3,4-dihydroxy 2-butanone 4-phosphate synthase
285
107
77
-0.47
0.2292
2.5.1.49
K01740 O-acetylhomoserine (thiol)-lyase
126
10
27
1.43
0.2304
3.5.2.14
K01474 N-methylhydantoinase B
445
35
94
1.43
0.2304
1.2.1.8
K00130 betaine-aldehyde dehydrogenase
76
6
16
1.42
0.2326
5.3.1.9
K01810 glucose-6-phosphate isomerase
154
12
32
1.42
0.2326
2.6.1.7
cysteine conjugate-beta lyase 2
43
15
11
-0.45
0.2349
3.2.1.31
K01195 beta-glucuronidase
42
15
11
-0.45
0.2349
6.1.1.1
K01866 tyrosyl-tRNA synthetase
397
145
106
-0.45
0.2349
4.4.1.16
selenocysteine lyase
239
19
50
1.4
0.2372
1.8.98.1
K03388 heterodisulfide reductase subunit A
819
296
218
-0.44
0.2377
1.1.1.103
L-threonine 3-dehydrogenase
69
24
18
-0.42
0.2434
1.13.11.52
K00463 indoleamine 2,3-dioxygenase
23
8
6
-0.42
0.2434
6.1.1.7
alanyl-tRNA synthetase
633
225
168
-0.42
0.2434
2.7.7.27
K00975 glucose-1-phosphate adenylyltransferase
287
24
61
1.35
0.2489
3.4.19.1
K01303 acylaminoacyl-peptidase
177
15
38
1.34
0.2512
3.4.21.92
K01358 ATP-dependent Clp protease, protease subunit
361
30
76
1.34
0.2512
2.4.2.29
K00773 queuine tRNA-ribosyltransferase
431
148
113
-0.39
0.252
6.1.1.11
K01875 seryl-tRNA synthetase
386
134
102
-0.39
0.252
2.1.1.72
K06223 DNA adenine methylase
1521
128
322
1.33
0.2536
2.1.1.14
361
122
94
-0.38
0.2549
217
74
57
-0.38
0.2549
163
14
35
1.32
0.256
2.5.1.19
K00549 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
K12960 5-methylthioadenosine/S-adenosylhomocysteine
deaminase
K00057 glycerol-3-phosphate dehydrogenase
(NAD(P)+)
K00800 3-phosphoshikimate 1-carboxyvinyltransferase
257
22
55
1.32
0.256
2.7.1.5
K00848 rhamnulokinase
72
6
15
1.32
0.256
3.4.16.4
K07260 D-alanyl-D-alanine carboxypeptidase
187
16
40
1.32
0.256
5.3.1.25
K01818 L-fucose isomerase
70
6
15
1.32
0.256
2.7.7.23
290
98
76
-0.37
0.2578
2.7.1.11
K04042 bifunctional UDP-N-acetylglucosamine
pyrophosphorylase / Glucosamine-1-phosphate Nacetyltransferase
K00850 6-phosphofructokinase
246
21
52
1.31
0.2584
1.2.1.3
K00128 aldehyde dehydrogenase (NAD+)
417
140
109
-0.36
0.2607
2.1.1.80
K00575 chemotaxis protein methyltransferase CheR
723
243
189
-0.36
0.2607
2.3.1.1
K00619 amino-acid N-acetyltransferase
286
95
74
-0.36
0.2607
1.2.1.38
256
22
54
1.3
0.2608
2.7.1.40
K00145 N-acetyl-gamma-glutamyl-phosphate/N-acetylgamma-aminoadipyl-phosphate reductase
K00873 pyruvate kinase
303
26
64
1.3
0.2608
2.8.1.1
K01011 thiosulfate/3-mercaptopyruvate sulfurtransferase
174
15
37
1.3
0.2608
6.3.5.4
K01953 asparagine synthase (glutamine-hydrolysing)
368
32
78
1.29
0.2632
4.1.2.13
K01623 fructose-bisphosphate aldolase, class I
563
188
147
-0.35
0.2636
1.4.1.1
alanine dehydrogenase
149
48
38
-0.34
0.2665
4.3.2.1
K01755 argininosuccinate lyase
295
97
77
-0.33
0.2694
3.5.1.25
K01443 N-acetylglucosamine-6-phosphate deacetylase
111
10
24
1.26
0.2705
6.3.5.3
K01952 phosphoribosylformylglycinamidine synthase
595
53
127
1.26
0.2705
3.5.4.1.1.1.94
2.4.1.12
K00694 cellulose synthase (UDP-forming)
89
8
19
1.25
0.2729
4.2.1.113
K02549 O-succinylbenzoate synthase
87
8
19
1.25
0.2729
2.4.2.2
K00756 pyrimidine-nucleoside phosphorylase
66
6
14
1.22
0.2803
6.3.2.12
224
71
58
-0.29
0.2811
6.3.2.17
K11754 dihydrofolate synthase / folylpolyglutamate
synthase
K01930 folylpolyglutamate synthase
224
71
58
-0.29
0.2811
6.5.1.1
K01971 DNA ligase (ATP)
371
116
95
-0.29
0.2811
1.10.2.2
ubiquinol-cytochrome c reductase hinge protein-like
174
16
37
1.21
0.2828
2.4.2.21
K00768 nicotinate-nucleotide--dimethylbenzimidazole
phosphoribosyltransferase
K01791 UDP-N-acetylglucosamine 2-epimerase
76
7
16
1.19
0.2878
226
21
48
1.19
0.2878
57
18
15
-0.26
0.2899
3.5.1.81
K00290 saccharopine dehydrogenase (NAD+, L-lysine
forming)
K06015 N-acyl-D-amino-acid deacylase
57
18
15
-0.26
0.2899
6.3.5.1
NAD synthetase
221
67
56
-0.26
0.2899
1.1.1.81
K00050 hydroxypyruvate reductase
126
12
27
1.17
0.2929
1.17.4.1
K00526 ribonucleoside-diphosphate reductase beta chain
1028
312
262
-0.25
0.2929
2.7.7.8
K00962 polyribonucleotide nucleotidyltransferase
378
36
81
1.17
0.2929
5.3.3.2
K01823 isopentenyl-diphosphate delta-isomerase
167
16
36
1.17
0.2929
6.1.1.17
K01885 glutamyl-tRNA synthetase
348
33
74
1.17
0.2929
6.2.1.3
K01897 long-chain acyl-CoA synthetase
846
256
215
-0.25
0.2929
6.3.5.10
K02232 adenosylcobyric acid synthase
125
12
27
1.17
0.2929
6.1.1.14
K01879 glycyl-tRNA synthetase beta chain
652
62
139
1.16
0.2954
6.1.1.12
K01876 aspartyl-tRNA synthetase
466
139
118
-0.24
0.2959
1.2.1.11
K00133 aspartate-semialdehyde dehydrogenase
298
29
64
1.14
0.3005
2.7.13.3
1679
163
360
1.14
0.3005
5.3.1.23
K07636 two-component system, OmpR family,
phosphate regulon sensor histidine kinase PhoR
K08963 methylthioribose-1-phosphate isomerase
309
30
66
1.14
0.3005
2.1.2.10
K00605 aminomethyltransferase
266
78
67
-0.22
0.3018
2.6.1.16
K00820 glucosamine--fructose-6-phosphate
aminotransferase (isomerizing)
604
178
153
-0.22
0.3018
5.1.3.14
1.5.1.7
3.6.4.6
K06027 vesicle-fusing ATPase
429
125
108
-0.21
0.3048
3.6.3.14
F0F1 ATP synthase subunit epsilon
1767
174
379
1.12
0.3057
1.4.1.13
573
164
144
-0.19
0.3108
1.4.3.4
K00265 glutamate synthase (NADPH/NADH) large
chain
K00274 monoamine oxidase
70
7
15
1.1
0.3108
6.5.1.4
K01974 RNA 3'-terminal phosphate cyclase
139
14
30
1.1
0.3108
6.2.1.16
K01907 acetoacetyl-CoA synthetase
79
8
17
1.09
0.3134
2.1.2.1
K00600 glycine hydroxymethyltransferase
436
125
110
-0.18
0.3137
6.3.2.9
220
62
55
-0.17
0.3167
2.2.1.7
K01925 UDP-N-acetylmuramoylalanine--D-glutamate
ligase
1-deoxy-D-xylulose-5-phosphate synthase
240
67
60
-0.16
0.3197
3.5.2.7
K01468 imidazolonepropionase
109
11
23
1.06
0.3213
6.1.1.20
K01889 phenylalanyl-tRNA synthetase alpha chain
775
216
194
-0.15
0.3228
3.6.3.27
549
57
118
1.05
0.3239
2.6.1.52
K02036 phosphate transport system ATP-binding
protein
K00831 phosphoserine aminotransferase
80
22
20
-0.14
0.3258
6.3.4.13
K01945 phosphoribosylamine--glycine ligase
324
34
70
1.04
0.3265
1.1.1.38
227
24
49
1.03
0.3292
4.2.3.5
K00027 malate dehydrogenase (oxaloacetatedecarboxylating)
K01736 chorismate synthase
297
79
73
-0.11
0.3348
1.1.5.3
sn-glycerol-3-phosphate dehydrogenase subunit C
229
25
50
1
0.3372
2.6.1.82
K09251 putrescine aminotransferase
53
6
12
1
0.3372
3.4.11.2
K01256 aminopeptidase N
165
18
36
1
0.3372
3.6.3.29
158
17
34
1
0.3372
2.7.7.7
K02017 molybdate transport system ATP-binding
protein
K02338 DNA polymerase III subunit beta
2735
721
675
-0.1
0.3378
3.4.21.66
K08651 thermitase
58
15
14
-0.1
0.3378
3.6.3.16
K01551 arsenite-transporting ATPase
289
76
71
-0.1
0.3378
5.99.1.3
K02470 DNA gyrase subunit B
1690
184
366
0.99
0.3399
2.4.2.11
K00763 nicotinate phosphoribosyltransferase
310
82
77
-0.09
0.3409
2.6.1.9
K00817 histidinol-phosphate aminotransferase
333
87
82
-0.09
0.3409
6.5.1.2
K01972 DNA ligase (NAD+)
290
32
63
0.98
0.3426
1.6.5.3
K00330 NADH dehydrogenase I subunit A
4484
1170
1104
-0.08
0.3439
6.1.1.19
arginyl-tRNA synthetase
444
115
109
-0.08
0.3439
3.5.1.18
K01439 succinyl-diaminopimelate desuccinylase
253
28
55
0.97
0.3453
3.5.4.26
K11752
diaminohydroxyphosphoribosylaminopyrimidine
deaminase / 5-amino-6-(5-phosphoribosylamino)uracil
reductase
K13953 alcohol dehydrogenase, propanol-preferring
165
43
41
-0.07
0.3469
743
83
161
0.96
0.348
170
19
37
0.96
0.348
4.4.1.11
K00590 site-specific DNA-methyltransferase (cytosineN4-specific)
K01761 methionine-gamma-lyase
91
23
22
-0.06
0.3499
4.3.1.19
K01754 threonine dehydratase
238
27
52
0.95
0.3507
2.3.2.2
K00681 gamma-glutamyltranspeptidase
230
26
50
0.94
0.3534
1.11.1.5
K00428 cytochrome c peroxidase
95
11
21
0.93
0.3561
2.6.1.19
274
32
60
0.91
0.3616
1.1.1.23
K07250 4-aminobutyrate aminotransferase / (S)-3amino-2-methylpropionate transaminase
K00013 histidinol dehydrogenase
295
73
72
-0.02
0.3621
3.6.4.13
K05592 ATP-dependent RNA helicase DeaD
278
33
61
0.89
0.3671
4.1.2.9
K01621 phosphoketolase
59
7
13
0.89
0.3671
6.3.1.2
K01915 glutamine synthetase
558
66
122
0.89
0.3671
3.4.11.1
K01255 leucyl aminopeptidase
241
59
59
0
0.3682
3.6.4.3
K13254 spastin
79
19
19
0
0.3682
4.1.3.39
K01666 4-hydroxy 2-oxovalerate aldolase
25
6
6
0
0.3682
1.1.1.14
K00008 L-iditol 2-dehydrogenase
141
17
31
0.87
0.3727
1.3.99.1
succinate dehydrogenase flavoprotein subunit
752
90
164
0.87
0.3727
4.2.1.120
4-hydroxyphenylacetate-3-hydroxylase (HpaA-1)
50
6
11
0.87
0.3727
4.99.1.3
K03795 sirohydrochlorin cobaltochelatase
48
6
11
0.87
0.3727
4.1.1.31
K01595 phosphoenolpyruvate carboxylase
292
70
71
0.02
0.3743
5.1.1.1
K01775 alanine racemase
261
62
63
0.02
0.3743
1.1.1.1
2.1.1.113
1.4.4.2
K00282 glycine dehydrogenase subunit 1
620
147
150
0.03
0.3774
2.5.1.15
K00796 dihydropteroate synthase
244
30
54
0.85
0.3783
4.2.1.49
K01712 urocanate hydratase
167
39
40
0.04
0.3804
3.2.1.4
K01179 endoglucanase
320
39
70
0.84
0.3811
4.1.99.1
K01667 tryptophanase
75
9
16
0.83
0.3839
1.1.1.18
K00010 myo-inositol 2-dehydrogenase
110
25
26
0.06
0.3865
3.4.23.42
K01385 thermopsin
95
22
23
0.06
0.3865
1.4.1.3
K00261 glutamate dehydrogenase (NAD(P)+)
336
42
74
0.82
0.3867
2.7.9.1
K01006 pyruvate,orthophosphate dikinase
403
50
88
0.82
0.3867
5.4.2.8
K00966 mannose-1-phosphate guanylyltransferase
352
44
77
0.81
0.3895
6.1.1.22
asparaginyl-tRNA synthetase
284
36
63
0.81
0.3895
6.1.1.6
lysyl-tRNA synthetase
488
61
107
0.81
0.3895
6.3.5.2
GMP synthase
519
65
114
0.81
0.3895
6.3.4.4
K01939 adenylosuccinate synthase
336
77
81
0.07
0.3896
5.4.99.2
K01849 methylmalonyl-CoA mutase, C-terminal domain
556
70
122
0.8
0.3923
5.4.2.1
K01834 phosphoglycerate mutase
719
163
172
0.08
0.3926
1.1.1.261
K00096 glycerol-1-phosphate dehydrogenase [NAD(P)]
117
15
26
0.79
0.3951
2.3.1.179
K09458 3-oxoacyl-[acyl-carrier-protein] synthase II
311
40
69
0.79
0.3951
1.2.1.70
K02492 glutamyl-tRNA reductase
195
43
46
0.1
0.3987
6.3.5.9
K02224 cobyrinic acid a,c-diamide synthase
132
29
31
0.1
0.3987
1.8.1.4
K00382 dihydrolipoamide dehydrogenase
480
62
106
0.77
0.4008
3.4.17.19
K01299 carboxypeptidase Taq
206
27
46
0.77
0.4008
4.1.2.27
K01634 sphinganine-1-phosphate aldolase
53
12
13
0.12
0.4049
1.2.1.16
167
22
37
0.75
0.4065
2.4.1.21
K00135 succinate-semialdehyde dehydrogenase
(NADP+)
K00703 starch synthase
339
73
80
0.13
0.4079
3.4.11.14
peptidase T
52
11
12
0.13
0.4079
1.14.99.30
zeta carotene desaturase candidate
45
6
10
0.74
0.4094
2.3.1.29
K00639 glycine C-acetyltransferase
135
18
30
0.74
0.4094
3.4.11.4
peptidase T
44
6
10
0.74
0.4094
6.1.1.3
K01868 threonyl-tRNA synthetase
498
66
110
0.74
0.4094
6.2.1.26
K01911 O-succinylbenzoic acid--CoA ligase
66
9
15
0.74
0.4094
3.6.4.12
K03655 ATP-dependent DNA helicase RecG
2293
493
544
0.14
0.411
3.5.3.12
K10536 agmatine deiminase
43
9
10
0.15
0.414
2.4.1.25
K00705 4-alpha-glucanotransferase
280
38
62
0.71
0.418
1.2.1.2
K00123 formate dehydrogenase, alpha subunit
660
139
156
0.17
0.4202
2.2.1.6
K01652 acetolactate synthase I/II/III large subunit
610
128
144
0.17
0.4202
2.6.1.87
uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose
diphosphate) aminotransferase, PLP-dependent
K01668 tyrosine phenol-lyase
230
49
55
0.17
0.4202
40
8
9
0.17
0.4202
211
29
47
0.7
0.4209
2.3.1.9
K00974 tRNA nucleotidyltransferase (CCA-adding
enzyme)
K00626 acetyl-CoA C-acetyltransferase
773
161
182
0.18
0.4232
2.7.7.6
K03040 DNA-directed RNA polymerase subunit alpha
2627
361
584
0.69
0.4238
3.1.4.3
K01114 phospholipase C
32
7
8
0.19
0.4263
4.3.1.1
K01744 aspartate ammonia-lyase
69
14
16
0.19
0.4263
1.3.1.34
K13237 peroxisomal 2,4-dienoyl-CoA reductase
74
10
16
0.68
0.4267
2.1.1.79
K00574 cyclopropane-fatty-acyl-phospholipid synthase
70
10
16
0.68
0.4267
4.2.1.20
K01696 tryptophan synthase beta chain
718
100
160
0.68
0.4267
1.1.1.35
K07516 3-hydroxyacyl-CoA dehydrogenase
240
34
54
0.67
0.4296
2.7.1.30
K00864 glycerol kinase
159
22
35
0.67
0.4296
6.3.3.1
235
33
52
0.66
0.4325
3.2.1.3
K01933 phosphoribosylformylglycinamidine cycloligase
K01178 glucoamylase
97
14
22
0.65
0.4354
4.1.2.5
K01620 threonine aldolase
98
14
22
0.65
0.4354
4.2.1.79
2-methylcitrate dehydratase
100
14
22
0.65
0.4354
6.1.1.5
K01870 isoleucyl-tRNA synthetase
763
108
170
0.65
0.4354
2.3.1.16
K07513 acetyl-CoA acyltransferase 1
183
37
43
0.22
0.4355
4.1.99.2
2.7.7.72
2.4.1.129
K05364 peptidoglycan glycosyltransferase
657
132
154
0.22
0.4355
5.4.2.7
K01839 phosphopentomutase
61
9
14
0.64
0.4383
2.4.1.1
K00688 starch phosphorylase
396
57
88
0.63
0.4413
5.4.99.16
K05343 maltose alpha-D-glucosyltransferase
56
11
13
0.24
0.4416
6.1.1.9
K01873 valyl-tRNA synthetase
690
136
161
0.24
0.4416
3.4.24.57
K01409 O-sialoglycoprotein endopeptidase
333
48
74
0.62
0.4442
4.2.3.4
K01735 3-dehydroquinate synthase
204
30
46
0.62
0.4442
6.3.5.7
1149
167
257
0.62
0.4442
1.3.99.2
K03330 glutamyl-tRNA(Gln) amidotransferase subunit
E
K00248 butyryl-CoA dehydrogenase
401
79
94
0.25
0.4446
5.5.1.4
K01858 myo-inositol-1-phosphate synthase
269
53
63
0.25
0.4446
1.3.99.3
K00249 acyl-CoA dehydrogenase
546
106
127
0.26
0.4477
5.99.1.2
K03168 DNA topoisomerase I
568
111
133
0.26
0.4477
1.1.1.157
K00074 3-hydroxybutyryl-CoA dehydrogenase
252
37
56
0.6
0.4501
2.7.7.19
K00970 poly(A) polymerase
304
45
68
0.6
0.4501
3.6.1.3
K01509 adenosinetriphosphatase
673
129
157
0.28
0.4538
2.6.1.44
108
16
24
0.58
0.456
3.4.13.9
K00827 alanine-glyoxylate transaminase / (R)-3-amino2-methylpropionate-pyruvate transaminase
K01271 Xaa-Pro dipeptidase
199
30
45
0.58
0.456
1.17.7.1
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
203
31
46
0.57
0.4589
1.13.11.5
K00451 homogentisate 1,2-dioxygenase
64
12
15
0.32
0.466
1.2.7.1
ferredoxin oxidoreductase alpha subunit
1069
199
248
0.32
0.466
2.3.1.15
K13508 glycerol-3-phosphate acyltransferase
260
48
60
0.32
0.466
2.3.3.1
K01647 citrate synthase
394
73
91
0.32
0.466
5.5.1.1
K01856 muconate cycloisomerase
42
8
10
0.32
0.466
4.2.1.2
K01679 fumarate hydratase, class II
458
71
103
0.54
0.4678
6.1.1.4
K01869 leucyl-tRNA synthetase
793
123
179
0.54
0.4678
1.1.1.193
K00082 5-amino-6-(5-phosphoribosylamino)uracil
reductase
K00058 D-3-phosphoglycerate dehydrogenase
235
43
54
0.33
0.469
632
116
146
0.33
0.469
1.1.1.95
1.1.1.85
K00052 3-isopropylmalate dehydrogenase
262
41
59
0.53
0.4708
2.7.7.63
K03800 lipoate-protein ligase A
269
49
62
0.34
0.472
1.1.1.42
K00031 isocitrate dehydrogenase
365
58
83
0.52
0.4738
1.5.3.1
N-methyltryptophan oxidase
191
30
43
0.52
0.4738
1.14.13.81
120
19
27
0.51
0.4767
3.4.21.102
K04035 magnesium-protoporphyrin IX monomethyl
ester (oxidative) cyclase
K03797 carboxyl-terminal processing protease
341
54
77
0.51
0.4767
2.7.2.3
phosphoglycerate kinase
372
67
86
0.36
0.4781
5.2.1.8
K03768 peptidyl-prolyl cis-trans isomerase B
(cyclophilin B)
CTP synthetase
445
80
103
0.36
0.4781
514
83
117
0.5
0.4797
173
31
40
0.37
0.4811
95
15
21
0.49
0.4827
871
141
198
0.49
0.4827
4.2.1.39
K01929 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6diaminopimelate--D-alanyl-D-alanine ligase
K00021 hydroxymethylglutaryl-CoA reductase
(NADPH)
K02434 aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln)
amidotransferase subunit B
hypothetical protein
59
10
13
0.38
0.4842
5.4.2.2
K01835 phosphoglucomutase
181
32
42
0.39
0.4872
1.1.1.205
K00088 IMP dehydrogenase
387
64
88
0.46
0.4917
1.1.1.47
K00034 glucose 1-dehydrogenase
53
9
12
0.42
0.4963
1.6.1.2
K00324 NAD(P) transhydrogenase subunit alpha
158
27
36
0.42
0.4963
6.3.4.2
6.3.2.10
1.1.1.34
6.3.5.6